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首页> 外文OA文献 >A surface active protein involved in aerial hyphae formation in the filamentous fungus Schizophillum commune restores the capacity of a bald mutant of the filamentous bacterium Streptomyces coelicolor to erect aerial structures
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A surface active protein involved in aerial hyphae formation in the filamentous fungus Schizophillum commune restores the capacity of a bald mutant of the filamentous bacterium Streptomyces coelicolor to erect aerial structures

机译：在丝状真菌Schizophillum commune中参与气生菌丝形成的表面活性蛋白恢复了丝状细菌天蓝色链霉菌的秃头突变体竖立空中结构的能力。

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The filamentous bacterium Streptomyces coelicolor undergoes a complex process of morphological differentiation involving the formation of a dense lawn of aerial hyphae that grow away from the colony surface into the air to form an aerial mycelium, Bald mutants of S, coelicolor, which are blocked in aerial mycelium formation, regain the capacity to erect aerial structures when exposed to a small hydrophobic protein called SapB, whose synthesis is temporally and spatially correlated with morphological differentiation. We now report that SapB is a surfactant that is capable of reducing the surface tension of water from 72 mJ m(-2) to 30 mJ m(-2) at a concentration of 50 mu g ml(-1) We also report that SapB, like the surface-active peptide streptofactin produced by the species S. tendae, was capable of restoring the capacity of bald mutants of S. tendae to erect aerial structures. Strikingly, a member (SC3) of the hydrophobin family of fungal proteins involved in the erection of aerial hyphae in the filamentous fungus Schizophyllum commune was also capable of restoring the capacity of S, coelicolor and S, tendae bald mutants to erect aerial structures. SC3 is unrelated in structure to SapB and streptofactin but, like the streptomycetes proteins, the fungal protein is a surface active agent. Scanning electron microscopy revealed that aerial structures produced in response to both the bacterial or the fungal proteins were undifferentiated vegetative hyphae that had grown away from the colony surface but had not commenced the process of spore formation, We conclude that the production of SapB and streptofactin at the start of morphological differentiation contributes to the erection of aerial hyphae by decreasing the surface tension at the colony surface but that subsequent morphogenesis requires additional developmentally regulated events under the control of bald genes.
机译：丝状细菌天蓝色链霉菌经历了复杂的形态分化过程，包括形成一个密集的空中菌丝草坪，该菌丝从菌落表面生长到空气中，形成空中菌丝体，天蓝色的S秃头突变体，天蓝色细菌被阻止在空中。当暴露于称为SapB的疏水小蛋白时，菌丝体的形成可恢复其架空结构的能力，该蛋白的合成在时间和空间上与形态分化有关。我们现在报告SapB是一种表面活性剂，能够在50μg ml（-1）的浓度下将水的表面张力从72 mJ m（-2）降低到30 mJ m（-2）。 SapB，就像由腱鞘菌种产生的表面活性肽链霉菌素一样，能够恢复腱鞘菌的秃头突变体竖立空中结构的能力。令人惊讶的是，参与在丝状真菌裂褶菌属中气生菌丝的建立的真菌蛋白疏水蛋白家族的成员（SC3）也能够恢复S，coelicolor和S，tena秃头突变体建立空中结构的能力。 SC3在结构上与SapB和链霉菌素无关，但像链霉菌蛋白质一样，真菌蛋白质是一种表面活性剂。扫描电子显微镜显示，响应细菌或真菌蛋白而产生的空中结构是未分化的营养菌丝，其已从菌落表面长出，但尚未开始孢子形成过程。我们得出结论，SapB和strofofactin的产生在形态分化的开始通过降低菌落表面的表面张力来促进气生菌丝的建立，但是随后的形态发生需要在秃头基因的控制下进行其他发育调控的事件。

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